Douglas Costa and Danilo have been ruled out of Brazil's last World Cup group game against Serbia due to injury.
The duo will not travel with the rest of the squad for Wednesday's closing Group E clash in Moscow.
Danilo started Brazil's 1-1 opening draw against Switzerland but missed their 2-0 win over Costa Rica due to a hip problem, with Fagner deputising.
Costa was influential off the bench as late goals from Philippe Coutinho and Neymar downed Costa Rica on Friday.
But the Juventus winger will not be available for the Serbia match as he has a hamstring injury, team doctor Rodrigo Lasmar confirmed.
"At the end of yesterday's game, Douglas Costa complained of pain in his right hamstring," Lasmar told reporters. "Just remember he arrived injured, but in the left leg. That is different from this complaint.
"He was diagnosed with a minor muscle injury so he will not travel with us. He will stay in Sochi, where we have a structure suitable for him to recover as soon as possible.
"We'll have a physiotherapist here. He will continue the recovery process here, along with Danilo, who will not travel and will have physiotherapy aimed at recovery.
"We are optimistic about his participation in the competition, depending on our performance at the World Cup. We are sparing no effort so can he still help us.
"The case that is most worrying is Douglas Costa's, but Danilo's is not much different, except that his is a less important, secondary muscle, so there's a little better expectation with respect to him."
Lasmar, though, is not willing to speculate on when Costa may be ready to return to action, with Brazil needing a point against Serbia to guarantee a last-16 spot.
"We are not going to need a date when he will be ready," Costa said.
"It is clear he is not in condition for this game and his recovery will tell us. We are imagining within a favourable time in the competition."
|Hart ready to leave City to revive career|
|Puel shuts down Schmeichel transfer speculation|
|Four-way tie at Barbasol Championship as Lincicombe misses cut|
|Lenglet happy not to face Messi|
|Tuchel keen to get PSG stars back after friendly defeat|
|Pulisic signing would interest Klopp|
|Whatever b******t you say, no one forgets – Klopp defends spending comments|
|Usyk outclasses Gassiev to make cruiserweight history|
|Pella books Cecchinato meeting in Umag, Ramanathan´s Newport run continues|
|Kisner expects nothing but exchange of ´horror stories´ with housemate Spieth|
|Inter new boy Lautaro Martinez denies Zenit in six-goal thriller|
|McIlroy eyeing early gains to apply scoreboard pressure|
|The Open 2018 diary: Spieth´s close shave|
|Salah is fully recovered and full of joy – Klopp|
|De Laurentiis accuses Sarri of trying to sign whole Napoli team|
|Lewis Hamilton explains German GP woes|
|O´Driscoll hails ´Test match animal´ Warburton|
|Spieth relishing prospect of Tiger tussle at Carnoustie|
|Sanches pushing for Bayern second chance - Kovac|
|Put on the Sunday red and enjoy Tiger´s ride regardless of Carnoustie outcome|
|Flawless Spieth takes share of Open lead after Tiger winds back the years|
|PSG´s Tuchel to be patient with World Cup stars Neymar, Mbappe & Cavani|
|Sanchez joins United tour in LA after visa issues|
|Catalans crush Salford to confirm Super League safety|
|Hamilton not giving up despite Hockenheim heartbreak|
|Spieth surges into Open Championship lead|
|Dumoulin is our biggest threat – Froome|
|Sane: Low gave me good reasons for World Cup snub|
|Tiger Woods in the hunt and loving it at The Open|
|Vettel delighted with German GP pole performance|
|Deschamps lauds intelligence of ´extraordinary´ Mbappe|
|Thomas maintains advantage as Fraile powers to stage victory|
|Woods sets clubhouse target, Kisner edges back in front|
|Bayern Munich 3 Paris Saint-Germain 1: Sanches strikes in ICC clash|
|Dost re-signs with Sporting CP after initially rescinding contract|
|Impressive Lions see off Jaguares to seal Waratahs semi|
|I´ll show Mourinho I´m ready, says United´s Pereira|
|Fognini sets up final clash with Gasquet|
|Injury ends Bouchard´s Swiss hopes, Sevastova through in Bucharest|
|That didn´t Work-u...race leader pulls up short of 3000metre finish|
|Prowling Tiger Woods on Open charge as Spieth joins leaders|
|Federer will struggle away from Wimbledon - Henman|
|Hamilton expecting tough Sunday at Hockenheim|
|Spalletti expects Icardi, Perisic to stay at Inter|
|Vettel smashes lap record to take Hockenheim pole after Hamilton woe|
|Warner targets Australia recall after Darwin debut|
|Rose: Open Sunday will be ´interesting´ after 64|
|Hamilton out of German GP qualifying as Mercedes falters|
|Pogba, Mbappe, Griezmann? Deschamps mulls Ballon d´Or contenders|
|Farah concussed on 250th Wests game as Tigers stun Souths|
|Waratahs overrun Highlanders in stunning second-half fightback|
|Rose scores record-equalling 64 to jump into Open contention|
|Moreno hails Liverpool´s recruitment drive as ´top, top players´ arrive|
|AC Milan elect new board with CEO Fassone out|
|Sri Lanka grind sorry South Africa into the Colombo dirt|
|Mina ready to reject offers for Barcelona stay|
|Guardiola tells Sane to use World Cup snub as motivation|
|Golovin´s ex-team-mate Chepchugov drops hint over Chelsea move|
|Reed & Rose make early charges on Carnoustie ´moving day´|
|Sancho ´had a point to prove´ in Dortmund´s win over Manchester City|
|Li hits back at AC Milan owners Elliott as bitter split continues|
|Crusaders cruise past Sharks to book Hurricanes semi-final|
|Rabiot would be a great signing for Barcelona – Xavi|
|JaVale McGee: Lakers are going to be ´one of the most fun teams ever´|
|Dolphins owner Ross: National anthem policy a ´placeholder´|
|Boga leaves Chelsea for Sassuolo|
|Schurrle in talks to leave Borussia Dortmund|
|Guardiola happy with Mahrez and Man City despite loss|
|Dodgers top Brewers in Machado´s debut|
|Raptors GM apologises to DeRozan after Spurs trade|